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KMID : 0360220180590060543
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Journal of the Korean Ophthalmological Society
2018 Volume.59 No. 6 p.543 ~ p.548
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Effect of Valproic Acid on Nitric Oxide and Nitric Oxide Synthase in Trabecular Meshwork Cell
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Son Myung-Seo
Park Cheul-Ho
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of valproic acid on the production of nitric oxide (NO) and expression of endothelial nitric oxide synthase (eNOS) in cultured human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to 0.25, 0.5, and 1.0 mM valproic acid for 6, 12, and 24 hours. Expression of eNOS mRNA was assessed with Reverse transcription-polymerase chain reaction, and production of NO was assessed with Griess assay. Cellular survival was assessed with the 3-[4, 5?dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay.
Results: Valproic acid at concentrations of 0.25, 0.5, 1.0 mM did not affect the cellular survival of HTMC significantly after exposure for 24 hours. Valproic acid increased NO production in a dose- and time-dependent manner. Also, valproic acid increased the degree of eNOS mRNA expression in a dose-dependent manner in HTMC.
Conclusions: Valproic acid increases production of NO and expression of eNOS mRNA in HTMC. Thus, valproic acid might increase aqueous outflow through the trabecular meshwork.
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KEYWORD
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Endothelial nitric oxide synthase (eNOS), Nitric oxide, Trabecular meshwork, Valproic acid
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